Abstract:

In this study, to improve the understanding of aroma characteristics, functional components, and nutritional variations in coriander at different growth stages, metabolomic approaches were employed to evaluate the profiles of volatile and non-volatile metabolites in coriander microgreens (CM) and coriander baby greens (CBG), focusing on their types and quantities. A comprehensive analysis identified 1,000 volatile and 1,799 non-volatile metabolites, among which 257 volatile and 597 non-volatile differential metabolites were recognized as key metabolites associated with coriander at various stages of maturity. Notably, key volatile organic compounds (VOCs) 1-p-menthen-8-thiol, 2-isobutyl-3-methoxypyrazine, 5-ethyl-3-hydroxy-4-methyl-2(5H)-furanone and β-ionone were identified as the primary odor determinants during the CM and CBM stages. Additionally, flavonoids such as kaempferol, quercetin, myricetin, isorhamnetin-3-O-glucoside, 7,4′-dihydroxyflavone significantly contribute to flavonoid biosynthesis pathways of coriander. Subsequently, a network pharmacology analysis was conducted to explore the potential therapeutic targets and pathways associated with the bioactive constituents pertinent to type 2 diabetes mellitus (T2DM) and obesity (OB), as identified through the metabolomics analysis. Through comprehensive bioactive ingredient screening, target prediction, protein–protein interaction network analysis, biological process examination, signaling pathway elucidation, and molecular docking validation, the underlying mechanisms by which CM and CBG potentially confer protection against T2DM and OB were elucidated. Through network pharmacology analysis, 10 core targets and their corresponding 49 compounds, including 17 flavonoids, were screened. This study establishes a theoretical framework for the application of CM and CBG as plant-based functional foods, distinguished by their flavor profiles, nutritional content, and health-promoting properties.