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大麦亲本材料性状鉴定及多样性分析
2016-10-19 11:59   审核人:

大麦亲本材料性状鉴定及多样性分析

Identification of Agoronomic Traits of Barley Parental Materials and Their Diversity Analysis

  

DOI10.7606/j.issn.1009-1041.2012.03.005

中文关键词:  大麦  SSR  遗传多样性  分子标记

英文关键词:Barley  Simple sequence repeats (SSR)  Genetic diversity  Molecular marker

基金项目:国家支撑计划项目(2007BAD52B08);国家自然基金项目(30771331);农业部大麦青稞产业技术体系岗位(CARS XX);甘肃省科技厅科技人员服务企业行动项目(SQ2009GJC 1005175)。

作者:王鹏喜, 勇,孟亚雄,李葆春,马小乐,王化俊 

(1.甘肃省作物遗传改良与种质创新重点实验室/甘肃省干旱生境作物学重点实验室甘肃兰州 7300702. 甘肃农业大学农学院甘肃兰州 730070 3.甘肃农业大学生命科学技术学院甘肃兰州 730070 

摘要:  为了给大麦育种亲本组配提供理论依据,对44个大麦亲本进行SSR标记分析并鉴定其农艺性状,结果表明,44个大麦材料的农艺性状存在不同程度差异,株高最高的为苏盐3号,最矮的为E14SCARLETT的穗型较理想。SSR分析结果表明,分布于大麦1H~7H染色体的32SSR位点有23个位点(71.88%)具有多态性。这32个位点共检测到127个等位变异,每个SSR位点能检测到1~6个,平均为3.9个。品种间遗传相似系数(GS)变幅为0.457~0.984,平均值为0.720。聚类分析表明,这32SSR标记能将44个亲本材料相互区分开,在GS0.610水平上可以聚为2个大类。

英文摘要:  In order to provide useful information for parent combination, the genetic diversity among 44 barley (Hordeum valgare L.) parental materials was investigated by using 32 simple sequence repeats (SSR) markers and some agronomic traits were also identified.There were some differences in some agronomic traits. The plant height of Sunyan3 was the highest, E14 was the lowest and could be used as lodging resistanto parent. And the panicle type of SCARLETT was ideal. The results indicated that 23 of the 32 SSR locus (71.88%) on 7 chromosomes were polymorphic. Among these 32 SSR locus, a total of 127 alleles were detected and 1 to 6 alleles could be detected at each SSR locus with an average of 3.9. Based on the SSR data, the genetic similarity (GS) among the 44 materials was calculated and ranged from 0.457 to 0.984, with the mean GS of 0.720. Clustering analysis showed that these parental materials could be distinguished by SSR markers.

麦类作物学报,2012,32(3):410-414
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